Molecular Screening of β-Lactoglobulin Gene Variation for Allergy-free Milk Production in Indonesian Friesian Holstein

Abstract The most allergenic protein in cow’s milk for new-borns is Beta Lactoglobulin (β-LG), which also one of the bovine whey proteins. As a result, choosing suitable animals to create free essential. In this study, Indonesian Friesian Holstein’s genetic variants β-LG gene were examined). Forty-two blood samples sampled as DNA sources. allele and genotype variant identified using PCR-RFLP, or Polymerase Chain Reaction - Restriction Fragment Length Polymorphism. Successful amplification with 247 bp product size was followed by sequencing HaeIII enzyme digestion (GG|CC). There discovered be three genotypes two types alleles, A B, frequencies being AA (0.26), AB (0.50), BB (0.24). population equilibrium because B have respective 0.51 0.49. conclusion, molecular-assisted selection can allergen-free utilizing genes.